Figure 1 Relative susceptibilities of A. polyphaga (open circles 24 h) and (open squares 96 h) to the following: (A) cholamphenicol (no IC50 indicated), (B) fusidic acid (IC50 at 0.125 % for 24 h and 0.062 % for 96 h), (C) framycetin sulphate (IC50s at 2.5% for 96 h), (D) gramicidin (IC50 at 0.05 for 96 h), (E) ciprofloxacin (no IC50 indicated), (F) benzalkonium cholride (no IC50 indicated), (G) phenylmercuric nitrate (no IC50 indicated) and (H) sodium carboxymethylcellulase (no IC50 indicated). A. polyphaga to each of the eye drop excipients tested (P < 0.05). Acanthamoeba cell numbers were assessed by measuring the percent alamarBlue reduction relative to that for the untreated control cultures [CON] over 24 and 96 h. The results are expressed as the means for triplicate cultures ±SEs.

%AlmarBlue Reduction relative to untreated Control cultures